As mentioned previously, Bio Basic Europe attended the ninth edition of the event “Cosmetorama” organized by the Hellenic Society of Cosmetology and held on the 22nd March in Athens Greece, which this year was dedicated to the claim substantiation topic. We showed to the public two scientific reports, concerning respectively the in-vivo efficacy test area, and the in-vitro analysis one. We desire to sum up as follows the main contents of these presentations, together with the relevant activities that our team of qualified professionals is able to perform. • IN VITRO TEST AS AN INNOVATIVE INSTRUMENT TO ASSESS THE SAFETY AND EFFICACY IN SUPPORT OF NEW GENERATION CLAIMS – Dr.ssa Eliana Regola (In Vitro Test Responsible) We presented three particular in vitro tests (1 safety test, 2 efficacy tests) performed within our laboratory (Bio Basic Lab – Parco Tecnico Scientifico of Pavia University) to evaluate the safety and efficacy of cosmetic products/medical devices, supporting new generation claims. SAFETY TEST - ALTERNATIVE METHOD TO EVALUATE THE PRO-SENSITISING ACTION OF COSMETIC PRODUCTS/MEDICAL DEVICES It is a morphological evaluation of THP-1 cells, performed with a cytofluorimetry analysis (Light scatter signals analysis (FSC/SSC)), which allows to discriminate damaged cells from the ones with a normal morphology. Substances characterized by known pro-sensitising properties, are able to cause in the cells morphological modifications, consequently the evaluation of the cell morphology appears a rapid and easy method to identify the pro-sensitising action of cosmetic product/medical device. This evaluation can be performed as a preliminary screening or to support the method normally used (OECD 442E-In Vitro Skin Sensitisation: human Cell Line Activation Test (h-CLAT)) EFFICACY TESTS - EVALUATION OF THE MUCO-ADHESIVENESS OF OPHTHALMIC PREPARATIONS ON RECONSTRUCTED CORNEAL EPITHELIUM Through a colorimetric reaction (spectrophotometric evaluation) we are able to quantify the accessible/not accessible sites for the mucus-adhesion on reconstructed corneal epithelium, and, consequently, to evaluate the efficacy of ophthalmic preparations in increasing the time of permanence on the epithelium, growing the bioavailability of the active principles contained therein. - ANTI-POLLUTION TEST In vitro evaluation, in terms of cell viability, of the protective and repairing action of a cosmetic product, towards environmental factors (UV, cigarette smoke, urban pollution and/or heavy metals standards) both on cell coltures and on reconstructed skin models. We are developing within our laboratory some protocols to evaluate those actions, through an assessment of the modulation of interleukins production and modulation/neutralization of free radicals, both on cell coltures and on reconstructed skin models. Our team of the In-Vitro Test Area is always available to provide you with more information. For further references: Technical Director Dr. Claudio Angelinetta In Vitro Test Responsible Dr.ssa Eliana Regola +39024155729 testing@biobasiceurope.it Thank you for your attention, BIO BASIC EUROPE S.r.l. Managing Director Dr. Umberto Pianca