IN VITRO EFFICACY TESTS

EVALUATION OF THE ABILITY OF A SAMPLE TO INCREASE THE SAMPLE ION OF SPECIFIC MARKER (COLLAGEN, ELASTIN, HYALURONIC ACID ...) IN CELL CULTURES
Aim of the test is to evaluate the ability of a cosmetic sample to modulate the synthesis of specific markers (collagen, elastin, hyaluronic acid ...) in cultures of keratinocytes / fibroblasts treated with the sample to be tested.
IN VITRO EVALUATION OF ANTIOXYDANT ACTIVITY
Aim of test is to assess if the tested sample, at different concentrations, is able to prevent the sample ion of reactive oxygen species (ROS) (ANTIOXIDANT ACTIVITY)
IN VITRO EVALUATION OF SCAVENGER ACTIVITY
Aim of the test is to evaluate if the tested sample, at different concentrations, is able to neutralize the reactive oxygen species (ROS free radicals).
IN VITRO EVALUATION OF THE REGENERATING ACTIVITY
Aim of the test is to assess the ability of a cosmetic sample to stimulate cell regeneration of fibroblasts/Keratinocytes or other cellular lines in vitro.
EVALUATION OF THE INHIBITION OF HYALURONIDASE
Aim of the test is the evaluation of the capacity of the sample to be tested to inhibit the activity of the enzyme hyaluronidase.
The inhibition of Hyaluronidase activity prevents rapid degradation of hyaluronic acid and is functional for maintaining the structural integrity of the cell membrane. Any inhibitors of the enzyme, maintaining the homeostasis of hyaluronic acid, could act as anti-aging factors.

EVALUATION OF PROTECTIVE ACTION AGAINST DAMAGE INDUCED BY ACTIVATION OF MMP-1
Aim of the test is to evaluate the ability of the test sample to protect cell cultures against the damage induced by the activation of MMP-1.
IN VITRO EVALUATION OF LIPOREDUCING ACTIVITY
Aim of the test is to determine the liporeducing activity of a cosmetic sample both by volumetric assessment of adipocytes (morphological analysis) and by measuring the variation of accumulation of triglycerides by the adipocytes.
IN VITRO EVALUATION OF VOLUMIZING ACTIVITY
Aim of the test is to determine the volumizing activity of a cosmetic sample both by volumetric assessment of adipocytes (morphological analysis) and by measuring the variation of accumulation of triglycerides by the adipocytes.
IN VITRO EVALUATION OF MUCOADESIVITY THROUGH IN VITRO TEST ON MUCOSAL CELLS AND/OR RECONSTRUCTED HUMAN MUCOSA MODELS
The test is performed on cell cultures of the mucosa and/or on reconstructed human mucosa models to evaluate the mucoadhesive in vitro of the tested sample . Mucoadhesiveness is determined by assessing the percentage of inhibition of lectin-glycoprotein binding in mucosal cells and/or on human mucosa models reconstructed in vitro.
ABSORPTION TEST ON HUMAN RECONSTRUCTED TISSUES AND ASSESSMENT OF THE EFFICIENCY OF SPECIFIC MARKER AFTER ABSORPTION
Aim of the test is to evaluate, in vitro, the absorption of specific markers, contained in a sample, through reconstructed human tissues and, if necessary, to verify the maintenance of the specific efficacy of the markers after absorption by in vitro efficacy tests on cell cultures.
ABSORPTION TEST THROUGH A CELLULAR MONOSTRATE OF INTESTINAL CELLS (Caco2) AND ASSESSMENT OF THE EFFICIENCY OF SPECIFIC MARKER AFTER ABSORPTION
Aim of the test is to evaluate, in vitro, the absorption of specific markers, contained in a sample, through a monolayer of intestinal cells (Caco-2) and, possibly, to verify the maintenance of the efficacy of the markers themselves after absorption by means of tests in vitro of efficacy on cell cultures.
EVALUATION OF THE PERCUTANEOUS ABSORPTION (OECD 428)
Aim of the test is to define the kinetics of dermal permeation (stratum corneum, vital epidermis and dermis) and transdermal of active substances through synthetic membranes or isolated skin by the use of the Franz cell.