IN VIVO SAFETY TESTS

SKIN IRRITATION TEST - Patch Test
Evaluation of the potential irritant effect of a cosmetic product according to the amended Draize method.
SKIN IRRITATION TEST - photopatch test
Evaluation of the potential irritant effect of a cosmetic product exposed to UVA and UVB radiations according to the amended Draize method.
SKIN IRRITATION TEST - Open Patch Test
Evaluation of the potential irritant effect of a cosmetic product according to the amended Draize method.
HUMAN REPEAT INSULT PATCH TEST (96 HOURS)
Evaluation of the potential irritant effect of a cosmetic product and its potential sensitizing action.
HYPOALLERGENICITY TEST
Evaluation of the potential of a cosmetic product to induce irritation or sensitization. This test is performed with repeated epicutaneous tests according to the amended Shelanski and Shelanski test (and its acceptability).
PHOTO-HYPOALLERGENICITY TEST
Evaluation of the potential irritating or sensitizing power of a cosmetic product exposed to UVA and UVB radiations with repeated epicutaneous tests according to the amended Shelanski and Shelanski test.
HRIPT – HUMAN REPEAT INSULTED PATCH TEST
Dermatological Evaluation of Topical Compatibility
Primary and Accumulated Dermal Irritation and
Dermal Sensitivity

Determination of the sun protection factor SPF (ISO 24444)
This test, carried out on 10 volunteers, allows the labeling of SPF of the tested product.
The test is based on the erythemic response of the skin to ultraviolet (UV) radiation obtained from a solar simulator with an arcuate lamp of xenon (or equivalent) of defined and known output.
It provides the value of SPF as the ratio between the energies required to induce a minimum erytheemic response on the protected skin (MEDp) and not (MEDu) with the tested product.
Generally, as a first step, the test is carried out on 5 volunteers, and in case of negative results, it will be possible to stop it at this preliminary level. On the contrary, if results are good the test will be completed on the remaining 5 volunteers and it will be written only this definitive report.

EVALUATION OF THE TOLERABILITY AND FUNCTIONALITY OF A PRODUCT WITH AN IN-USE TEST MONITORED BY A GYNECOLOGIST
This test is performed for those products that are generally used in contact with the vaginal area in order to assess the tolerability on external vaginal skin and mucosa and its functionality.
IN VIVO DETERMINATION OF THE SUN PROTECTION FACTOR (SPF) (UNI EN ISO 24444:2011) + EVALUATION OF WATER RESISTANCE (COLIPA GUIDELINES FOR EVALUATING SUN PRODUCT WATER RESISTANCE, DECEMBER 2005)
This test, carried out on 10 volunteers, allows the labeling of SPF and to verify the water resistant activity of the tested product.
Generally, as a first step, the test is carried out on 5 volunteers, and in case of negative results, it will be possible to stop it at this preliminary level. On the contrary, if results are good the test will be completed on the remaining 5 volunteers and it will be written only this definitive report.

IN VIVO DETERMINATION OF THE SUN PROTECTION FACTOR (SPF) (UNI EN ISO 24444:2011) + EVALUATION OF VERY WATER RESISTANCE (COLIPA GUIDELINES FOR EVALUATING SUN PRODUCT WATER RESISTANCE, DECEMBER 2005)
This test, carried out on 10 volunteers, allows the labeling of SPF and to verify the very water resistant activity of the tested product.
Generally, as a first step, the test is carried out on 5 volunteers, and in case of negative results, it will be possible to stop it at this preliminary level. On the contrary, if results are good the test will be completed on the remaining 5 volunteers and it will be written only this definitive report.

CLINICAL TEST: EVALUATION OF THE NON COMEDOGENICITY
The scope of this test is to evaluate if the use of the investigated cosmetic product does not induce the occurrence of new closed and open comedones and/or does not worsen the status of comedones.
OPHTHALMIC TEST: EVALUATION OF THE OCULAR TOLERABILITY
The aim of this test is to evaluate if the investigated cosmetic product is well tolerated in the eye contour.